Technical Note, Sample Pooling for Microarray Analysis: A Statistical Assesment of Risks and Biases
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چکیده
Pooling of RNA samples isolated from tissue is a strategy that can be implemented in microarray experiments when the amount of sample RNA is limiting, or when variation across samples must be reduced. However, it is widely documented that important gene expression information is lost during pooling. This Technical Note qualifies and quantifies the effects of pooling on biological conclusions derived from gene expression data.
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Biases induced by pooling samples in microarray experiments
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